Journal: Cell Reports Medicine

Article Title: Tissue-specific transcriptional profiles and heterogeneity of natural killer cells and group 1 innate lymphoid cells

doi: 10.1016/j.xcrm.2022.100812

Figure Lengend Snippet: Inter-tissue-specific signatures of NK and ILC1 subpopulations (A) Transcriptome-based clusters computed for each organ separately, projected onto the multi-organ integrated UMAP obtained with Harmony. (B) Heatmap representing overlap coefficient dissimilarity between transcriptome-based clusters calculated separately for each organ. (C) Dot plot of NK cell-specific versus ILC1-specific markers. Intermediate clusters were not taken into account. (D) Dot plot of specific markers for each cluster. (E) Flow cytometry profiles of CD49a, DNAM-1, PD-1H, and SDC4 expression, analyzed in Lin − NKp46 + NK1.1 + cells. Histograms represent the frequency of CD49a − CD49b + NK cells and CD49a + CD49b − ILC1s for each indicated marker across organs. Data are shown as mean ± SEM and are pooled from two independent experiments. Each point represents a pool of two mice.

Article Snippet: APC anti-mouse SDC4 (REA640) , Miltenyi Biotec , Cat# 130-109-831; RRID: AB_2653641.

Techniques: Flow Cytometry, Expressing, Marker

Journal: Cell Reports Medicine

Article Title: Tissue-specific transcriptional profiles and heterogeneity of natural killer cells and group 1 innate lymphoid cells

doi: 10.1016/j.xcrm.2022.100812

Figure Lengend Snippet:

Article Snippet: APC anti-mouse SDC4 (REA640) , Miltenyi Biotec , Cat# 130-109-831; RRID: AB_2653641.

Techniques: Recombinant, Saline, Staining, Cell Isolation, Sensitive Assay, Software

Journal: The Journal of Cell Biology

Article Title: αv-Class integrin binding to fibronectin is solely mediated by RGD and unaffected by an RGE mutation

doi: 10.1083/jcb.202004198

Figure Lengend Snippet: Integrin expression. (a and b) FACS-based measurements of integrin levels on the surface of Fn1 +/+ and Fn1 ΔRGD/ΔRGD ES cells (a) and integrin and syndecan 4 levels on Fn1 +/+ and Fn1 ΔRGD/ΔRGD fibroblasts (b). Intensities obtained with specific antibodies were normalized to isotype controls. Intensity values near 1 indicate that similar values were obtained with specific antibody and the isotype control. Raw cells were used as positive control for α4 integrin expression. Experiments were repeated three times, and 10,000 cells were analyzed per experiment. AU, arbitrary units. (c) Quantitative real-time PCR analysis of Itga8 gene expression in Fn1 ΔRGD fibroblasts with mRNA from mouse lung as a positive control. Data are from three independent experiments performed in triplicate (normalized to GAPDH). Bars represent mean ± SD of the average fold induction compared with mouse lung tissue. Statistical significances were calculated using two-tailed unpaired Student t tests; *, P < 0.05; ***, P < 0.001.

Article Snippet: For flow cytometry, the following antibodies conjugated to PE were used: hamster anti-mouse β1 integrin (1:200; 102207; BioLegend), rat anti-mouse α5 integrin (1:200; 557447; PharMingen), hamster anti-mouse β3 integrin (1:200; 12–0611; BD Biosciences), rat anti-mouse αv integrin (1:200; 551187; PharMingen), rat anti-mouse α4 integrin (1:200; 01271D; PharMingen), and unconjugated rat anti-mouse syndecan 4 (1:200; 550350; PharMingen) together with mouse anti-rat FITC (10094D; PharMingen).

Techniques: Expressing, Positive Control, Real-time Polymerase Chain Reaction, Two Tailed Test